SubtiBank SubtiBank
truB
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truB

tRNA pseudouridine 55 synthase
Locus
BSU_16660
Isoelectric point
6.65
Molecular weight
34.05 kDa
Protein length
309 aa Sequence Blast
Gene length
930 bp Sequence Blast
Function
tRNA modification
Product
tRNA pseudouridine 55 synthase
Essential
no
Synonyms
ylxN,ymxF

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,736,886 1,737,815

The protein

Catalyzed reaction/ biological activity

  • uridine55 in tRNA --> pseudouridine55 in tRNA (according to UniProt)
  • Protein family

  • pseudouridine synthase TruB family (single member, according to UniProt)
  • Structure

  • 1ZE1 (from Thermotoga maritima, 36% identity) PubMed
  • Biological materials

    Mutant

  • BKE16660 (truB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGCCTTTCACTCCTTC, downstream forward: _UP4_CAATAGAAAAAAGGTGACCG
  • BKK16660 (truB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGCCTTTCACTCCTTC, downstream forward: _UP4_CAATAGAAAAAAGGTGACCG
  • References

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