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treP
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treP

trehalose permease of the phosphotransferase system, EIIBC of the PTS
Locus
BSU_07800
Isoelectric point
8.56
Molecular weight
49.84 kDa
Protein length
470 aa Sequence Blast
Gene length
1413 bp Sequence Blast
Function
trehalose uptake and phosphorylation
Product
trehalose-specific PTS permease, EIIBC component
Essential
no
E.C.
2.7.1.69
Synonyms
treB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Phosphotransferase system → Sugar specific PTS proteins
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of trehalose
  • Groups of genes → Membrane proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on a Cys residue

    • Gene

    Coordinates
    850,367 → 851,779

    The protein

    Catalyzed reaction/ biological activity

  • α,α-trehalose + Nπ-phospho-L-histidyl-[protein] --> α,α-trehalose 6-phosphate + L-histidyl-[protein] (according to UniProt)

    • Protein family

  • PTS permease, sucrose family PubMed

    • Paralogous protein(s)

  • SacX, SacP, MurP, BglP

    • Domains

  • PTS EIIB domain type-1 (aa 1-88) (according to UniProt)
  • PTS EIIC domain type-1 (aa 108-470) (according to UniProt)

    • Structure

  • 2IPJ (from Clostridium difficile, the PTS EIIB domain, 41.4% identity) PubMed

    • Localization

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    treP-treA-treR
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • TreR: repression, PubMed, in TreR regulon
  • CcpA: repression, PubMed, in CcpA regulon
  • PhoP: activation, PubMed, in PhoP regulon

    • Regulation

  • expressed under conditions of phosphate limitation (PhoP) PubMed
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    Biological materials

    Mutant

  • MGNA-C259 (treP::erm), available at the NBRP B. subtilis, Japan
  • BKE07800 (ΔtreP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCAACCCCTCCGTATC, downstream forward: _UP4_TAACAAGTGGGGAGCGGGAC
  • BKK07800 (ΔtreP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCAACCCCTCCGTATC, downstream forward: _UP4_TAACAAGTGGGGAGCGGGAC

    • References

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