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thyB
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thyB

thymidylate synthase B
Locus
BSU_21820
Isoelectric point
6.27
Molecular weight
30.39 kDa
Protein length
264 aa Sequence Blast
Gene length
795 bp Sequence Blast
Function
biosynthesis of thymidine nucleotides
Product
thymidylate synthase B
Essential
no
E.C.
2.1.1.45
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of pyrimidine nucleotides
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

    • Gene

    Coordinates
    2,297,106 2,297,900

    The protein

    Catalyzed reaction/ biological activity

  • (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP --> 7,8-dihydrofolate + dTMP (according to UniProt)

    • Protein family

  • thymidylate synthase family (with ThyA, according to UniProt)

    • Paralogous protein(s)

  • ThyA

    • Modification

  • phosphorylated on Arg-102 PubMed

    • Structure

  • 3IX6 (from Brucella melitensis, 67% identity)

    • Expression and Regulation

    Operons

    Genes
    ypjP-ypjQ-thyB-dfrA-ypkP
    Description
    PubMed
    view in new tab

    Genes
    thyB-dfrA
    Description
    PubMed

    Regulatory mechanism

  • CcpN: activation, weak, in CcpN regulon

    • Regulation

  • weak induction in the presence of glucose (CcpN) PubMed
    • view in new tab

    Biological materials

    Mutant

  • BKE21820 (thyB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTTTCATCCTTTAAA, downstream forward: _UP4_GTATGATTTCATTCATTTTT
  • BKK21820 (thyB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTTTCATCCTTTAAA, downstream forward: _UP4_GTATGATTTCATTCATTTTT

    • References

    Reviews

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    Original publications

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