SubtiBank SubtiBank
thiO
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thiO

FAD-dependent glycine oxidase
Locus
BSU_11670
Isoelectric point
5.90
Molecular weight
40.78 kDa
Protein length
369 aa Sequence Blast
Gene length
1110 bp Sequence Blast
Function
biosynthesis of thiamine
Product
glycine oxidase
Essential
no
E.C.
1.4.3.19
Synonyms
goxB,yjbR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of thiamine

    • Gene

    Coordinates
    1,243,735 1,244,844

    The protein

    Catalyzed reaction/ biological activity

  • glycine + H2O + O2 --> glyoxylate + H2O2 + NH4+ (according to UniProt)
  • H2O + N-ethylglycine + O2 --> ethylamine + glyoxylate + H2O2 (according to UniProt)
  • H2O + O2 + sarcosine --> glyoxylate + H2O2 + methylamine (according to UniProt)
  • D-alanine + H2O + O2 --> H2O2 + NH4+ + pyruvate (according to UniProt)
  • glyphosate + H2O + O2 --> aminomethylphosphonate + glyoxylate + H+ + H2O2 (according to UniProt)

    • Protein family

  • DAO family (single member, according to UniProt)

    • Modification

  • FAD PubMed

    • Cofactors

  • FAD (according to UniProt)

    • Structure

  • 1NG3 (complex with acetyl-glycine), 1RYI

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    tenA-tenI-thiO-thiS-thiG-thiF-thiD
    Description
    PubMed

    Regulatory mechanism

  • Thi-box: RNA switch, via RNA switch, in Thi-box regulon

    • Regulation

  • repressed by thiamine (Thi-box) PubMed
  • the Thi-box RNA is degraded by RNase Y PubMed
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    Biological materials

    Mutant

  • MGNA-B164 (yjbR::erm), available at the NBRP B. subtilis, Japan
  • BKE11670 (thiO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATTCCGCCTCCAATCACCA, downstream forward: _UP4_CGCAAGGAGGCGGTTCAGAT
  • BKK11670 (thiO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATTCCGCCTCCAATCACCA, downstream forward: _UP4_CGCAAGGAGGCGGTTCAGAT

    • References

    Reviews

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    Original publications

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