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spoVD
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spoVD

mother-cell specific class B penicillin-binding protein (spore cortex)
Locus
BSU_15170
Isoelectric point
8.85
Molecular weight
71.08 kDa
Protein length
646 aa Sequence Blast
Gene length
1941 bp Sequence Blast
Function
synthesis of spore peptidoglycan
Product
penicillin-binding protein (spore cortex)
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall synthesis → Penicillin-binding proteins
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    1,584,214 1,586,154

    The protein

    Catalyzed reaction/ biological activity

  • synthesis of spore peptidoglycan PubMed
  • protects SpoVE from proteolytic degradation PubMed

    • Protein family

  • transpeptidase family (according to UniProt)

    • Paralogous protein(s)

  • PbpB

    • Domains

  • C-terminal PASTA domain (aa 580-638) PubMed

    • Effectors of protein activity

  • intramolecular disulfide bonds between two Cys residues are reduced by StoA, this is rquired for activity of SpoVD PubMed

    • Structure

  • 1PYY (PBP2B from Spreptococcus pneumoniae, 26% identity) PubMed

    • Localization

  • intermembrane space that separates forespores from mother cells PubMed
  • outer forespore membrane PubMed

    • Expression and Regulation

    Operons

    Genes
    spoVD
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

    • Regulatory mechanism

  • SpoIIID: repression, PubMed, in SpoIIID regulon

    • Regulation

  • expressed during sporulation in the mother cell (SigE) PubMed
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    Biological materials

    Mutant

  • 1A975 ( spoVD::cat), PubMed, available at BGSC
  • BKE15170 (spoVD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGAGACCGTTCACTCCTT, downstream forward: _UP4_TGATTCGGGCTGCCTATTCT
  • BKK15170 (spoVD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGAGACCGTTCACTCCTT, downstream forward: _UP4_TGATTCGGGCTGCCTATTCT

    • References

    Reviews

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    Original Publications

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