SubtiBank SubtiBank
spoIIIAH
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spoIIIAH

component of the SpoIIIA-SpoIIQ type III secretion system residing in the forespore membrane, required for SigG activation
Locus
BSU_24360
Isoelectric point
4.63
Molecular weight
23.58 kDa
Protein length
218 aa Sequence Blast
Gene length
657 bp Sequence Blast
Function
activation of SigG, forespore encasement by the spore coat
Product
part of the transmembrane channel linking the mother cell and the forespore
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Protein secretion
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    2,532,353 2,533,009

    Phenotypes of a mutant

  • reduced sporulation efficiency (1 to 10% compared to wild type) PubMed
  • the spoIIIL spoIIIAH double mutant has a severe sporulation defect (0.001%) PubMed
  • block of sporulation after engulfment

    • The protein

    Catalyzed reaction/ biological activity

  • required for forespore encasement by the spore coat PubMed

    • Structure

  • 3UZO; 3TUF (the SpoIIQ-SpoIIIAH pore forming complex) PubMed

    • Localization

  • membrane protein, forms a transmembrane channel linking the mother cell and the forespore (with SpoIIQ) PubMed
  • proper recruitment to the sporulation septum on the mother cell side requires SpoIIQ PubMed

    • Expression and Regulation

    Operons

    Genes
    yqhV-spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

    • Regulatory mechanism

  • SpoIIID: repression, PubMed, in SpoIIID regulon

    • Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

    • Additional information

  • the internal promoter is essential for sporulation, it is twice as active as the promoter in front of spoIIIAA, suggesting that SpoIIIAG and SpoIIIAH are required in larger amounts as compared to the other products of the operon PubMed
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    Genes
    spoIIIAG-spoIIIAH
    Description
    PubMed

    Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

    • Additional information

  • the internal promoter is essential for sporulation, it is twice as active as the promoter in front of spoIIIAA, suggesting that SpoIIIAG and SpoIIIAH are required in larger amounts as compared to the other products of the operon PubMed
    • view in new tab

    Genes
    spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH
    Description
    PubMed

    Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

    • Additional information

  • the internal promoter is essential for sporulation, it is twice as active as the promoter in front of spoIIIAA, suggesting that SpoIIIAG and SpoIIIAH are required in larger amounts as compared to the other products of the operon PubMed
    • view in new tab

    Biological materials

    Mutant

  • BKE24360 (spoIIIAH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCAAACGGTTTGTTTTTTAA, downstream forward: _UP4_TAAGAATGAGGGAAAAAAGC
  • BKK24360 (spoIIIAH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCAAACGGTTTGTTTTTTAA, downstream forward: _UP4_TAAGAATGAGGGAAAAAAGC

    • Labs working on this gene/protein

  • Charles Moran, Emory University, NC, USA homepage

    • References

    Reviews

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    Original publications

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