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spoIIIAG
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spoIIIAG

component of the SpoIIIA-SpoIIQ type III secretion system residing in the forespore membrane, required for SigG activation
Locus
BSU_24370
Isoelectric point
6.41
Molecular weight
25.34 kDa
Protein length
229 aa Sequence Blast
Gene length
690 bp Sequence Blast
Function
activation of SigG
Product
component of a type III secretion system
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Protein secretion
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    2,533,010 2,533,699

    The protein

    Structure

  • 5WC3 PubMed

    • Localization

  • membrane protein, in membranes that surround the forespore, this requires SpoIIIAH and SpoIIQ PubMed
  • forms an oligomeric ring-like conduit in the space between the mother cell and forespore membranes PubMed

    • Expression and Regulation

    Operons

    Genes
    yqhV-spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

    • Regulatory mechanism

  • SpoIIID: repression, PubMed, in SpoIIID regulon

    • Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

    • Additional information

  • the internal promoter is essential for sporulation, it is twice as active as the promoter in front of spoIIIAA, suggesting that SpoIIIAG and SpoIIIAH are required in larger amounts as compared to the other products of the operon PubMed
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    Genes
    spoIIIAG-spoIIIAH
    Description
    PubMed

    Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

    • Additional information

  • the internal promoter is essential for sporulation, it is twice as active as the promoter in front of spoIIIAA, suggesting that SpoIIIAG and SpoIIIAH are required in larger amounts as compared to the other products of the operon PubMed
    • view in new tab

    Genes
    spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH
    Description
    PubMed

    Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

    • Additional information

  • the internal promoter is essential for sporulation, it is twice as active as the promoter in front of spoIIIAA, suggesting that SpoIIIAG and SpoIIIAH are required in larger amounts as compared to the other products of the operon PubMed
    • view in new tab

    Biological materials

    Mutant

  • BKE24370 (spoIIIAG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTTATTCATTGCCGACACT, downstream forward: _UP4_AAAAAAATAAAGGAGGATTC
  • BKK24370 (spoIIIAG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTTATTCATTGCCGACACT, downstream forward: _UP4_AAAAAAATAAAGGAGGATTC

    • References

    Reviews

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    Original Publications

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