SubtiBank SubtiBank
sinI
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sinI

antagonist of SinR, drives SlrR from the SlrR(LOW) to the SlrR(HIGH) state
Locus
BSU_24600
Isoelectric point
6.33
Molecular weight
6.47 kDa
Protein length
57 aa Sequence Blast
Gene length
174 bp Sequence Blast
Function
control of biofilm formation
Product
antagonist of SinR
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProt

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of transcription factor (other than two-component system)
  • Information processing → Regulation of gene expression → Transition state regulators
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Regulation

    • Gene

    Phenotypes of a mutant

  • altered cell death pattern in colonies PubMed

    • The protein

    Paralogous protein(s)

  • SlrA

    • Domains

  • Sin domain (aa 2-40) (according to UniProt)

    • Structure

  • 5TMX PubMed
  • 1B0N (complex SinR-SinI) PubMed

    • Expression and Regulation

    Operons

    Genes
    sinI-sinR
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • Spo0A: activation, in a portion of cells PubMed, in Spo0A regulon
  • SinR: repression, PubMed, in SinR regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • ScoC: repression, PubMed, in ScoC regulon

    • Regulation

  • repressed during exponential growth (ScoC) PubMed

    • Additional information

  • the mRNA is stabilized upon depletion of RNase Y PubMed
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    Biological materials

    Mutant

  • GP959 (spc), available in Jörg Stülke's lab
  • DS91 (spc) NCIB3610 derivate, available in Jörg Stülke's lab
  • 1S98 ( sinI::kan), PubMed, available at BGSC
  • GP1663 (yqhG-sinI-sinR-tasA), available in Jörg Stülke's lab
  • BKE24600 (sinI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCAGTTTCTCCTCCTAA, downstream forward: _UP4_TGAATGTGCTATAATATCAC
  • BKK24600 (sinI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCAGTTTCTCCTCCTAA, downstream forward: _UP4_TGAATGTGCTATAATATCAC

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • References

    Reviews

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    Modelling of the SinI]/[[SinR] switch

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    Original publications

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