SubtiBank SubtiBank
rpsNB
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rpsNB

alternative ribosomal protein S14*, can replace RpsN under conditions of zinc limitation
Locus
BSU_08880
Isoelectric point
11.19
Molecular weight
10.20 kDa
Protein length
89 aa Sequence Blast
Gene length
270 bp Sequence Blast
Function
translation
Product
alternative ribosomal protein S14*
Essential
no
Synonyms
rpsNB,yhzA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → Ribosomal proteins

    • Gene

    Coordinates
    965,909 966,178

    The protein

    Protein family

  • universal ribosomal protein uS14 family (with RpsN, according to UniProt)

    • Paralogous protein(s)

  • RpsN

    • Structure

  • 5AFI (the E. coli ribosome, 47% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    rpsNB
    Description
    PubMed

    Regulatory mechanism

  • Zur: repression, in the presence of zinc PubMed, in Zur regulon
  • YlxR: regulation, PubMed, in YlxR regulon

    • Regulation

  • induced by zinc starvation (Zur) PubMed
  • part of the third wave of induction upon zinc depletion PubMed
  • expression requires functional YlxR PubMed
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    Additional information

  • the mRNA is very stable (> 15 min) PubMed

    • Biological materials

    Mutant

  • BKE08880 (rpsNB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGTCACATAGCCTCCCTT, downstream forward: _UP4_TGGTAAGAAAAAACTCCGGC
  • BKK08880 (rpsNB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGTCACATAGCCTCCCTT, downstream forward: _UP4_TGGTAAGAAAAAACTCCGGC

    • References

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