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rocD
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rocD

ornithine transaminase
Locus
BSU_40340
Isoelectric point
4.89
Molecular weight
43.60 kDa
Protein length
401 aa Sequence Blast
Gene length
1203 bp Sequence Blast
Function
arginine, ornithine and citrulline utilization
Product
ornithine transaminase
Essential
no
E.C.
2.6.1.13
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of arginine/ ornithine

    • Gene

    Coordinates
    4,144,301 → 4,145,506

    The protein

    Catalyzed reaction/ biological activity

  • 2-oxocarboxylate + L-ornithine --> L-α-amino acid + L-glutamate 5-semialdehyde (according to UniProt)

    • Protein family

  • Class-III pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

    • Cofactors

  • PLP (according to UniProt)

    • Structure

  • 3RUY (from B. anthracis, 76% identity, 93% similarity)

    • Localization

  • cytoplasm (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    rocD-rocE-rocF
    Description
    PubMed

    Sigma factors

  • SigL: sigma factor, PubMed, in SigL regulon

    • Regulatory mechanism

  • Spo0A: repression, PubMed, in Spo0A regulon
  • AhrC: activation, PubMed, in AhrC regulon
  • CodY: repression, PubMed, in CodY regulon
  • RocR: activation, PubMed, in RocR regulon

    • Regulation

  • induced by arginine (RocR, AhrC) PubMed

    • Additional information

  • expression of the rocD-rocE-rocF operon is increased upon depletion of nusA (resulting from increased ahrC expression) Reference
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    Biological materials

    Mutant

  • GP656, aphA3, available in Jörg Stülke's lab
  • BKE40340 (ΔrocD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTTGAATTCCCCCTTG, downstream forward: _UP4_TAAATCCGCACCATGGCGGG
  • BKK40340 (ΔrocD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTTGAATTCCCCCTTG, downstream forward: _UP4_TAAATCCGCACCATGGCGGG

    • References

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