phosphoribosylaminoimidazole carboxamide formyltransferase
function
purine biosynthesis
product
phosphoribosylaminoimidazole carboxamide formyltransferaseand inosine-monophosphate cyclohydrolase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]Gene
Coordinates
708,594 710,132
Phenotypes of a mutant
more resistant than wild-type to zinc limitation when grown on rich medium (LB), this is blocked by simultaneous deletion of [gene|29FF54E2B9F4A11811F5E15885A4C02A259C7B35|purB] (reason: accumulation of ZTP that stimulates the binding of [protein|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|ZagA] to [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|FolE], and thus folate biosynthesis during zinc limitation) [pubmed|31132310]The protein
Catalyzed reaction/ biological activity
(6S)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide --> (6S)-5,6,7,8-tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)H2O + IMP --> 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)Protein family
purH family (single member, according to UniProt)[SW|Domains]
MGS-like domain (aa 1-146) (according to UniProt)Structure
[PDB|1ZCZ] (from ''Thermotoga maritima'', 36% identity, 54% similarity) [Pubmed|18260100]additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]Biological materials
Mutant
BKE06520 ([gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAABKK06520 ([gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAAReferences
3036807,12923093,15378759,7638212,31132310