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pdhD
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pdhD

dihydrolipoamide dehydrogenase E3 subunit of both pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes
Locus
BSU_14610
Isoelectric point
4.76
Molecular weight
49.57 kDa
Protein length
470 aa Sequence Blast
Gene length
1413 bp Sequence Blast
Function
links glycolysis and TCA cycle, enzyme in TCA cycle
Product
dihydrolipoamide dehydrogenase E3 subunit of both pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes
Essential
no
E.C.
1.8.1.4
Synonyms
citL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Carbon core metabolism → TCA cycle
  • Groups of genes → Phosphoproteins → Phosphorylation on either a Ser, Thr or Tyr residue

    • Gene

    Coordinates
    1,531,870 → 1,533,282

    Phenotypes of a mutant

  • defects in sporulation and unable to grow on glucose as single carbon source PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • Protein N6-(dihydrolipoyl)lysine + NAD+ --> protein N6-(lipoyl)lysine + NADH2 (according to UniProt)

    • Protein family

  • class-I pyridine nucleotide-disulfide oxidoreductase family (with AcoL and LpdV, according to UniProt)

    • Paralogous protein(s)

  • LpdV, AcoL

    • Kinetic information

  • Michaelis-Menten PubMed

    • Modification

  • phosphorylated (Ser/Thr/Tyr) PubMed

    • Cofactors

  • FAD (according to UniProt)

    • Effectors of protein activity

  • Inhibited thiamine 2-thiothiazolone diphosphate and NADH PubMed
  • Low sensibility to NADPH PubMed

    • Structure

  • 1EBD (complex with binding domain of dihydrolipoamide acetylase, Geobacillus stearothermophilus), 1EBD (complex with binding domain of dihydrolipoamide acetylase, Geobacillus stearothermophilus)

    • Localization

  • cytoplasm (according to UniProt)

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    pdhA-pdhB-pdhC-pdhD
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • stringent response: negative regulation, due to presence of guanine at 1 position of the transcript PubMed, in stringent response

    • Regulation

  • pdhA: expression activated by glucose (1.9-fold) PubMed
    • view in new tab

    Genes
    pdhC-pdhD
    Description
    PubMed

    Regulation

  • pdhA: expression activated by glucose (1.9-fold) PubMed
    • view in new tab

    Biological materials

    Mutant

  • BKE14610 (ΔpdhD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGGGAAATCTCCTACTACCA, downstream forward: _UP4_TAATTTTCATATCAAAAACA
  • BKK14610 (ΔpdhD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AGGGAAATCTCCTACTACCA, downstream forward: _UP4_TAATTTTCATATCAAAAACA

    • LacZ fusion

  • pGP723 (in pAC5), available in Jörg Stülke's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • FLAG-tag construct

  • GP1427 (spc, based on pGP1331), available in the Jörg Stülke's lab

    • References

    Reviews

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    Original publications

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