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murQ
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murQ

N-acetyl-muramic acid-6P etherase (cleaves lactate from N-acetylmuramic acid)
Locus
BSU_01700
Isoelectric point
5.40
Molecular weight
32.55 kDa
Protein length
304 aa Sequence Blast
Gene length
915 bp Sequence Blast
Function
cell wall turnover
Product
N-acetyl-muramic acid-6P etherase
Essential
no
Synonyms
ybbI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Utilization of cell wall components
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of amino sugars
  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of amino sugars
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

    • Gene

    Coordinates
    192,051 192,965

    Phenotypes of a mutant

  • strongly reduced viability during prolonged incubation in stationary phase PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • H2O + N-acetyl-D-muramate 6-phosphate --> (R)-lactate + N-acetyl-D-glucosamine 6-phosphate (according to UniProt)

    • Protein family

  • GCKR-like family (single member, according to UniProt)

    • Domains

  • SIS domain (aa 59-222) (according to UniProt)

    • Modification

  • phosphorylation on Ser-2 PubMed

    • Structure

  • 4S12 (from Yersinia enterocolitica, 51% identity)

    • Expression and Regulation

    Operons

    Genes
    murQ-murR-murP-amiE-nagZ-ybbC
    Description
    PubMed

    Regulatory mechanism

  • MurR: repression, PubMed, in MurR regulon

    • Regulation

  • expressed in late exponential and early stationary phase PubMed
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    Biological materials

    Mutant

  • MGNA-B952 (ybbI::erm), available at the NBRP B. subtilis, Japan
  • BKE01700 (murQ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAGGATGCCCCCTGTTT, downstream forward: _UP4_CATCCATGATAAGGAGAGAA
  • BKK01700 (murQ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAGGATGCCCCCTGTTT, downstream forward: _UP4_CATCCATGATAAGGAGAGAA

    • References

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