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melR
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melR

transcriptional regulator of the melR-melE-melD-melC-melA operon
Locus
BSU_30260
Isoelectric point
6.45
Molecular weight
38.24 kDa
Protein length
344 aa Sequence Blast
Gene length
1035 bp Sequence Blast
Function
regulation of melibiose utilization
Product
transcriptional regulator (LacI family)
Essential
no
Synonyms
msmR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of melibiose
  • Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other

    • Gene

    Coordinates
    3,096,782 3,097,816

    The protein

    Catalyzed reaction/ biological activity

  • represses the the melR-melE-melD-melC-melA operon in the absence of melibiose or raffinose PubMed

    • Protein family

  • LacI family

    • Domains

  • HTH lacI-type domain (aa 2-58) (according to UniProt)

    • Structure

  • 1VPW (E. coli PurR, 29% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    melR-melE-melD-melC-melA
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • MelR: repression, PubMed, in MelR regulon

    • Regulation

  • induced by melibiose or raffinose (MelR) PubMed
  • repressed by glucose (CcpA) PubMed
  • there is an additional RNA "upshift" signal in front of the melE gene suggestive of a melE-melD-melC-melA mRNA PubMed. However, there is no promoter in front of melE, suggesting that this mRNA may be the product of mRNA processing PubMed
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    Biological materials

    Mutant

  • MGNA-A802 (msmR::erm), available at the NBRP B. subtilis, Japan
  • BKE30260 (melR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGGTATCCCTCTCATCT, downstream forward: _UP4_TAAGCAAAGATTCATCACGA
  • BKK30260 (melR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGGTATCCCTCTCATCT, downstream forward: _UP4_TAAGCAAAGATTCATCACGA

    • References

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