SubtiBank SubtiBank
moaE
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moaE

molybdopterin synthase (large subunit), catalyses the transfer of sulfide from MoaD thiocarboxylate
Locus
BSU_14300
Isoelectric point
4.75
Molecular weight
17.64 kDa
Protein length
157 aa Sequence Blast
Gene length
474 bp Sequence Blast
Function
nitrate respiration
Product
molybdopterin synthase (large subunit)
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,498,966 1,499,439

The protein

Catalyzed reaction/ biological activity

  • catalyses the transfer of sulfide from MoaD thiocarboxylate
  • 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-CH2-C(O)SH + cyclic pyranopterin phosphate + H2O --> 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 4 H+ + molybdopterin (according to UniProt)
  • Protein family

  • moaE family (single member, according to UniProt)
  • Structure

  • 1NVI (the MoaD-MoaE complex from E. coli) PubMed
  • Expression and Regulation

    Biological materials

    Mutant

  • BKE14300 (moaE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCGTAATCTCAAACCGTT, downstream forward: _UP4_CCAGACCTAAGCGAGGGAGA
  • BKK14300 (moaE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCGTAATCTCAAACCGTT, downstream forward: _UP4_CCAGACCTAAGCGAGGGAGA
  • References

    Reviews

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    Original publications

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