SubtiBank SubtiBank
mleN
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mleN

malate-H+/Na+-lactate antiporter
Locus
BSU_23560
Isoelectric point
7.25
Molecular weight
50.05 kDa
Protein length
468 aa Sequence Blast
Gene length
1407 bp Sequence Blast
Function
malate uptake
Product
malate-H+/Na+-lactate antiporter
Essential
no
Synonyms
yqkI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Transporters/ other → Transporter for organic acids
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of organic acids
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    2,452,800 2,454,206

    Phenotypes of a mutant

  • abrupt arrest in the mid-logarithmic phase of growth on malate when low concentrations of protonophore were present PubMed

    • The protein

    Protein family

  • NhaC Na(+)/H(+) (TC 2.A.35) antiporter family (with NhaC, according to UniProt)

    • Paralogous protein(s)

  • NhaC

    • Localization

  • membrane associated PubMed

    • Expression and Regulation

    Operons

    Genes
    mleN-mleA
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • AnsR: repression, PubMed, in AnsR regulon
  • CcpA: repression, PubMed, in CcpA regulon

    • Regulation

  • ansA: expressed in the presence of asparagine (AnsR) PubMed
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    Genes
    ansA-ansB-mleN-mleA
    Description
    PubMed

    Regulation

  • ansA: expressed in the presence of asparagine (AnsR) PubMed
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    Biological materials

    Mutant

  • MGNA-C411 (yqkI::erm), available at the NBRP B. subtilis, Japan
  • GP1460 (mleN::spc), available in Jörg Stülke's lab
  • BKE23560 (mleN::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAAGAAGTCCCCCTTCAT, downstream forward: _UP4_GGCTAATAGGGGAGGATCAC
  • BKK23560 (mleN::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAAGAAGTCCCCCTTCAT, downstream forward: _UP4_GGCTAATAGGGGAGGATCAC

    • LacZ fusion

  • pGP388 (in pAC7), available in Jörg Stülke's lab

    • References

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