SubtiBank SubtiBank
metQ
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

metQ

methionine ABC transporter (binding lipoprotein)
Locus
BSU_32730
Isoelectric point
8.49
Molecular weight
30.20 kDa
Protein length
274 aa Sequence Blast
Gene length
825 bp Sequence Blast
Function
methionine uptake
Product
methionine ABC transporter (binding lipoprotein)
Essential
no
Synonyms
yusA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Cellular processes → Transporters → ABC transporters → Importers → Uptake of amino acids
  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of methionine/ S-adenosylmethionine
  • Groups of genes → Secreted proteins
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    3,361,767 3,362,591

    The protein

    Protein family

  • nlpA lipoprotein family (with YhcJ, according to UniProt)

    • Paralogous protein(s)

  • YhcJ

    • Structure

  • 4GOT

    • Localization

  • associated to the cell membrane, via MetP PubMed
  • extracellular (signal peptide) PubMed, lipid modification as retention signal

    • Expression and Regulation

    Operons

    Genes
    metN-metP-metQ
    Description
    PubMed

    Regulatory mechanism

  • S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination, in S-box
  • CodY: activation, PubMed, in CodY regulon

    • Regulation

  • activated during growth in the presence of branched chain amino acids (CodY) PubMed
  • the mRNA is processed between metP and metQ by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed
  • the S-box RNA is degraded by RNase Y PubMed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A595 (yusA::erm), available at the NBRP B. subtilis, Japan
  • BKE32730 (metQ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TAGCTTTTTCAATGTAAATC, downstream forward: _UP4_TAAGACTGAAACCCCGGATG
  • BKK32730 (metQ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TAGCTTTTTCAATGTAAATC, downstream forward: _UP4_TAAGACTGAAACCCCGGATG

    • References

    Loading