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lonB

Lon-like ATP-dependent protease

Locus

BSU_28210

Isoelectric point

6.27

Molecular weight

60.26 kDa

Protein length

552 aa Sequence Blast

Gene length

1659 bp Sequence Blast

Function

protein quality control

Product

Lon-like ATP-dependent protease

Essential

Synonyms

ysxF

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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Categories containing this gene/protein

Information processing → Protein synthesis, modification and degradation → Proteolysis → Additional proteins involved in proteolysis

Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Groups of genes → Membrane proteins

Gene

Coordinates

2,882,971 → 2,884,629

The protein

Catalyzed reaction/ biological activity

Hydrolysis of proteins in presence of ATP (according to UniProt)

Protein family

Peptidase S16 family (with DdcP and LonA, according to UniProt)

Domains

Lon proteolytic (aa 349-535) (according to UniProt)

Structure

6U5Z (from E. coli, corresponds to aa 93 ... 538, 27% identity)

Localization

localized to the forespore membrane early in development, followed by localization throughout the forespore later in development PubMed

Expression and Regulation

Operons

Genes

lonB

Description

PubMed

Sigma factors

SigF: sigma factor, PubMed, in SigF regulon

Regulation

expressed early during sporulation in the forespore (SigF) PubMed

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Biological materials

Mutant

BKE28210 (ΔlonB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTGGTCCCTCCTGAAA, downstream forward: _UP4_TAAACCCTATTTTGATAATA

BKK28210 (ΔlonB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTGGTCCCTCCTGAAA, downstream forward: _UP4_TAAACCCTATTTTGATAATA

References

Reviews

Original publications