SubtiBank SubtiBank
lipC
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

lipC

spore coat protein, phospholipase implicated in spore germination
Locus
BSU_04110
Isoelectric point
5.84
Molecular weight
23.46 kDa
Protein length
213 aa Sequence Blast
Gene length
642 bp Sequence Blast
Function
spore germination
Product
spore coat phospholipase B
Essential
no
Synonyms
ycsK

Genomic Context

      
Loading

Categories containing this gene/protein

Gene

Coordinates
462,431 463,072

Phenotypes of a mutant

  • defective in L-alanine-stimulated germination PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • cleaves the fatty acids at the sn-1 and sn-2 positions of phospholipids PubMed
  • Protein family

  • 'GDSL' lipolytic enzyme family (according to UniProt)
  • Structure

  • 4PPY (from Bacteroides fragilis, corresponds to aa 41 ... 210, 25.4% identity)
  • Localization

  • spore coat (basement), localization depends on SpoIVA PubMed
  • Expression and Regulation

    Operons

    Genes
    Description
    (according to DBTBS) null

    Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon
  • Regulatory mechanism

  • GerE: activation, PubMed, in GerE regulon
  • Regulation

  • expressed four hours after onset of sporulation (GerE) PubMed
  • view in new tab

    Description

    Regulatory mechanism

  • KipR: repression, PubMed, in KipR regulon
  • TnrA: activation, PubMed, in TnrA regulon
  • Regulation

  • induced in the presence of 5-oxoproline PubMed
  • view in new tab

    Biological materials

    Mutant

  • MGNA-C024 (ycsK::erm), available at the NBRP B. subtilis, Japan
  • BKE04110 (lipC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTCTTTCCTCCACTCT, downstream forward: _UP4_TAGAAAACGCGCCTCATGAC
  • BKK04110 (lipC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTCTTTCCTCCACTCT, downstream forward: _UP4_TAGAAAACGCGCCTCATGAC
  • References

    Reviews

    Loading

    Original publications

    Loading