SubtiBank SubtiBank
gutB
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gutB

D-sorbitol dehydrogenase
Locus
BSU_06150
Isoelectric point
5.21
Molecular weight
38.23 kDa
Protein length
353 aa Sequence Blast
Gene length
1062 bp Sequence Blast
Function
glucitol utilization
Product
D-sorbitol dehydrogenase
Essential
no
E.C.
1.1.1.14
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of glucitol

    • Gene

    Coordinates
    667,466 668,527

    The protein

    Catalyzed reaction/ biological activity

  • D-fructose + H+ + NADH --> D-sorbitol + NAD+ (according to UniProt)
  • NAD+ + xylitol --> D-xylulose + H+ + NADH (according to UniProt)
  • L-iditol + NAD+ --> H+ + L-sorbose + NADH (according to UniProt)

    • Protein family

  • zinc-containing alcohol dehydrogenase family (according to UniProt)

    • Cofactors

  • NAD+ (according to UniProt)

    • Structure

  • 1PL7 (the human enzyme, 42% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    gutB-gutP-fruC
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • GutR: activation, PubMed, in GutR regulon

    • Regulation

  • induced by glucitol (GutR) PubMed
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    Biological materials

    Mutant

  • BKE06150 (gutB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGGCAAGTTCCTTTCT, downstream forward: _UP4_TGAGTGAACAGGGAGGATCT
  • BKK06150 (gutB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGGCAAGTTCCTTTCT, downstream forward: _UP4_TGAGTGAACAGGGAGGATCT

    • References

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