SubtiBank SubtiBank
yxaA
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yxaA

putative glycerate kinase
Locus
BSU_40040
Isoelectric point
4.53
Molecular weight
39.24 kDa
Protein length
382 aa Sequence Blast
Gene length
1149 bp Sequence Blast
Function
unknown
Product
putative glycerate kinase
Essential
no
E.C.
2.7.1.31
Synonyms
glxK

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
4,112,073 4,113,221

The protein

Catalyzed reaction/ biological activity

  • (R)-glycerate + ATP --> 3-phospho-D-glycerate + ADP + H+ (according to UniProt)
  • Protein family

  • glycerate kinase type-1 family (single member, according to UniProt)
  • Structure

  • 3CWC (from Salmonella Typhimurium 58% identity)
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed, in SigB regulon
  • Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon
  • Regulation

  • induced by stress (SigB) PubMed
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    Biological materials

    Mutant

  • MGNA-B680 (yxaA::erm), available at the NBRP B. subtilis, Japan
  • BKE40040 (yxaA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCAAATCGCCTCTTTCA, downstream forward: _UP4_TAGCCGATGCACATGCCAAT
  • BKK40040 (yxaA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCAAATCGCCTCTTTCA, downstream forward: _UP4_TAGCCGATGCACATGCCAAT
  • References

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