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glpD
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glpD

glycerol-3-phosphate dehydrogenase (menaquinone 7)
Locus
BSU_09300
Isoelectric point
8.00
Molecular weight
62.36 kDa
Protein length
555 aa Sequence Blast
Gene length
1668 bp Sequence Blast
Function
glycerol utilization
Product
glycerol-3-phosphate dehydrogenase (menaquinone 7)
Essential
no
E.C.
1.1.5.3
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of glycerol/ glycerol-3-phosphate
  • Metabolism → Lipid metabolism → Utilization of lipids → Utilization of phospholipids

    • Gene

    Coordinates
    1,004,975 → 1,006,642

    The protein

    Catalyzed reaction/ biological activity

  • quinone + sn-glycerol 3-phosphate --> quinol + dihydroxyacetone phosphate (according to UniProt)

    • Protein family

  • FAD-dependent glycerol-3-phosphate dehydrogenase family (single member, according to UniProt)

    • Cofactors

  • FAD (according to UniProt)

    • Structure

  • 3DA1 (from Bacillus halodurans, 57% identity, 72% similarity)

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    glpD
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpA: repression, in CcpA regulon
  • AbrB: activation, PubMed, in AbrB regulon
  • GlpP: antitermination, at a RNA switch PubMed, in GlpP regulon

    • Regulation

  • induced by glycerol (GlpP) PubMed
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    Biological materials

    Mutant

  • QB5347 (cat), available in the Stülke lab
  • BKE09300 (ΔglpD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTACGTTTCCTCCTTGTT, downstream forward: _UP4_TAAATCATAACGGGCTGTCT
  • BKK09300 (ΔglpD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTACGTTTCCTCCTTGTT, downstream forward: _UP4_TAAATCATAACGGGCTGTCT

    • References

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