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frlD
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frlD

fructosamine kinase
Locus
BSU_32570
Isoelectric point
4.91
Molecular weight
30.83 kDa
Protein length
284 aa Sequence Blast
Gene length
855 bp Sequence Blast
Function
metabolism of sugar amines
Product
fructosamine kinase
Essential
no
Synonyms
yurL

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,347,051 → 3,347,905

The protein

Protein family

  • carbohydrate kinase PfkB family (according to UniProt)
  • Structure

  • 1TYY (from Salmonella typhimurium, 24% identity) PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • CodY: repression, PubMed, PubMed, in CodY regulon
  • FrlR: repression, PubMed, in FrlR regulon
  • YlxR: regulation, PubMed, in YlxR regulon
  • Regulation

  • FrlB: repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • induced in the absence of YlxR PubMed
  • Additional information

  • the frlB-frlO-frlN-frlM-frlD-yurJ operon is strongly downregulated in a cshA mutant PubMed
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    Biological materials

    Mutant

  • MGNA-A558 (yurL::erm), available at the NBRP B. subtilis, Japan
  • BKE32570 (ΔfrlD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATCAATTTCATAGCGCTTC, downstream forward: _UP4_TAAGCAGTGTCTCGTTTTTT
  • BKK32570 (ΔfrlD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATCAATTTCATAGCGCTTC, downstream forward: _UP4_TAAGCAGTGTCTCGTTTTTT
  • References

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