SubtiBank SubtiBank
epsK
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epsK

export of extracellular poly-N-acetylglucosamine
Locus
BSU_34265
Isoelectric point
10.00
Molecular weight
55.00 kDa
Protein length
505 aa Sequence Blast
Gene length
1518 bp Sequence Blast
Function
export of extracellular poly-N-acetylglucosamine
Product
poly-N-acetylglucosamine exporter
Essential
no
Synonyms
yvfA,yvfB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Transporters/ other → Other exporters
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Matrix polysaccharide synthesis
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    3,517,485 3,519,002

    Phenotypes of a mutant

  • smooth colonies on MsGG medium, no biofilm formation PubMed

    • The protein

    Protein family

  • MOP exporter family

    • Localization

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • RemA: activation, PubMed, in RemA regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • SinR: anti-activation, (prevents binding of RemA) PubMed, in SinR regulon
  • EAR riboswitch: processive antitermination, in EAR riboswitch

    • Regulation

  • repressed by SinR PubMed

    • Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed
  • expression is increased in rsiX mutants PubMed
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    Biological materials

    Mutant

  • MGNA-B611 (yvfA::erm), available at the NBRP B. subtilis, Japan
  • BKE34265 (epsK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGCGCTGAAATTTATCGTGA, downstream forward: _UP4_AAAACGAAAGGAGCTGTGAA
  • BKK34265 (epsK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGCGCTGAAATTTATCGTGA, downstream forward: _UP4_AAAACGAAAGGAGCTGTGAA

    • Labs working on this gene/protein

  • Richard Losick, Harvard Univ., Cambridge, USA homepage

    • References

    Reviews

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    The EAR RNA switch

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