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epsB
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epsB

extracellular polysaccharide synthesis, protein tyrosine kinase, phosphorylation of EpsE
Locus
BSU_34360
Isoelectric point
9.92
Molecular weight
24.53 kDa
Protein length
227 aa Sequence Blast
Gene length
684 bp Sequence Blast
Function
biofilm formation
Product
protein tyrosine kinase
Essential
no
Synonyms
yveL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Protein modification → Protein kinases
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Matrix polysaccharide synthesis
  • Groups of genes → Phosphoproteins → Phosphorylation on a Tyr residue

    • Gene

    Coordinates
    3,528,462 3,529,145

    The protein

    Catalyzed reaction/ biological activity

  • phosphorylation of EpsE, to stimulate EpsE activity and biofilm formation PubMed
  • ATP + L-tyrosyl-[protein] --> ADP + H+ + O-phospho-L-tyrosyl-[protein] (according to UniProt)

    • Protein family

  • BY kinase, see the Bacterial Protein Tyrosine Kinase Database
  • CpsD/CapB family (with PtkA, according to UniProt)

    • Paralogous protein(s)

  • PtkA

    • Modification

  • autophosphorylated on Tyr-225 and Tyr-227 PubMed

    • Structure

  • 2VED (CapB, the homolog in Staphylococcus aureus) PubMed

    • Expression and Regulation

    Operons

    Genes
    epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • RemA: activation, PubMed, in RemA regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • SinR: anti-activation, (prevents binding of RemA) PubMed, in SinR regulon
  • EAR riboswitch: processive antitermination, in EAR riboswitch

    • Regulation

  • repressed by SinR PubMed

    • Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed
  • expression is increased in rsiX mutants PubMed
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    Biological materials

    Mutant

  • MGNA-A072 (yveL::erm), available at the NBRP B. subtilis, Japan
  • GP1518 (aphA3) PubMed, available in Jörg Stülke's lab
  • GP1519 (epsA-epsB, aphA3) PubMed, available in Jörg Stülke's lab
  • BKE34360 (epsB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTTTTTTCTAAAGATCACTC, downstream forward: _UP4_TAGTTTTTGTAAAGGTGATG
  • BKK34360 (epsB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTTTTTTCTAAAGATCACTC, downstream forward: _UP4_TAGTTTTTGTAAAGGTGATG

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH) PubMed, available in Jörg Stülke's lab

    • FLAG-tag construct

  • GP1541 epsB-FLAG 3x spc (based on pGP1331) available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Richard Losick, Harvard Univ., Cambridge, USA homepage

    • References

    Reviews

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    Original publications

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