SubtiBank SubtiBank
dhbA
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dhbA

2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
Locus
BSU_32000
Isoelectric point
5.31
Molecular weight
27.34 kDa
Protein length
261 aa Sequence Blast
Gene length
786 bp Sequence Blast
Function
biosynthesis of the siderophore bacillibactin
Product
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
Essential
no
E.C.
1.3.1.28
Synonyms
entA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Homeostasis → Acquisition of iron → Acquisition of iron / Other
  • Metabolism → Additional metabolic pathways → Iron metabolism → Acquisition of iron / Other
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Other proteins required for biofilm formation

    • Gene

    Coordinates
    3,291,511 3,292,296

    Phenotypes of a mutant

  • defective in biofilm formation, this can be suppressed by the addition of 2,3-dihydroxybenzoate to the medium PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + NAD+ --> 2,3-dihydroxybenzoate + H+ + NADH (according to UniProt)

    • Protein family

  • Short-chain dehydrogenases/reductases (SDR) family (according to UniProt)

    • Structure

  • 2FWM (from E. coli, 42% identity) PubMed

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    dhbA-dhbC-dhbE-dhbB-dhbF-ybdZ
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigI: sigma factor, PubMed, in SigI regulon

    • Regulatory mechanism

  • Fur: repression, PubMed, in Fur regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • Kre: repression, in Kre regulon

    • Regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed
  • expression of the operon is strongly induced at the beginning of biofilm formation PubMed

    • Additional information

  • the dhbA-dhbC-dhbE-dhbB-dhbF operon is strongly unregulated in a kre mutant PubMed
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    Biological materials

    Mutant

  • BKE32000 (dhbA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCATCAATTCCTTTCT, downstream forward: _UP4_TAACTGAATTTAAAGGAGGT
  • BKK32000 (dhbA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCATCAATTCCTTTCT, downstream forward: _UP4_TAACTGAATTTAAAGGAGGT

    • References

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