SubtiBank SubtiBank
cysH
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cysH

phosphoadenosine phosphosulfate sulfotransferase
Locus
BSU_15570
Isoelectric point
5.46
Molecular weight
26.83 kDa
Protein length
233 aa Sequence Blast
Gene length
702 bp Sequence Blast
Function
sulfate reduction
Product
phosphoadenosine phosphosulfate sulfotransferase
Essential
no
E.C.
1.8.4.8
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Additional metabolic pathways → Sulfur metabolism
  • Metabolism → Additional metabolic pathways → Sulfur metabolism → sulfur metabolism/ general

    • Gene

    Coordinates
    1,630,382 1,631,083

    The protein

    Catalyzed reaction/ biological activity

  • [thioredoxin]-disulfide + adenosine 3',5'-bisphosphate + 2 H+ + sulfite --> 3'-phosphoadenylyl sulfate + [thioredoxin]-dithiol (according to UniProt)

    • Protein family

  • PAPS reductase family (with YitB, according to UniProt)

    • Paralogous protein(s)

  • YitB

    • Structure

  • 2GOY (from Pseudomonas aeruginosa, 35% identity) PubMed

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    cysH-cysP-sat-cysC-ylnD-sirB-ylnF
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CymR: repression, in CymR regulon
  • S-box: termination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

    • Regulation

  • induced by methionine starvation (S-box) PubMed
  • the S-box RNA is degraded by RNase Y PubMed
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    Biological materials

    Mutant

  • BKE15570 (cysH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCTTCTTTCCTCCTTT, downstream forward: _UP4_CTGCATGAATAAGGAGCTGC
  • BKK15570 (cysH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCTTCTTTCCTCCTTT, downstream forward: _UP4_CTGCATGAATAAGGAGCTGC

    • Labs working on this gene/protein

  • [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France

    • References

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