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cypC
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cypC

long chain-fatty acid beta-hydroxylating cytochrome P450, H(2)O(2)-dependent, hydroxylates myristic acid to beta-hydroxymyristic acid, required for protection against paraquat stress
Locus
BSU_02100
Isoelectric point
6.47
Molecular weight
47.95 kDa
Protein length
417 aa Sequence Blast
Gene length
1254 bp Sequence Blast
Function
biosynthesis of beta-hydroxy fatty acid for lipopeptides
Product
fatty acid beta-hydroxylating cytochrome P450, protection against paraquat stress
Essential
no
Synonyms
ybdT
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Electron transport and ATP synthesis → Electron transport/ other
  • Metabolism → Lipid metabolism → Lipid metabolism/ other
  • Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
  • Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

    • Gene

    Coordinates
    229,525 230,778

    The protein

    Catalyzed reaction/ biological activity

  • hydroxylation of a long-chain fatty acid (e.g. myristic acid) at the alpha- and beta-positions using hydrogen peroxide as an oxidant PubMed
  • 1,2-saturated fatty acid + H2O2 --> 2-hydroxy fatty acid + H2O (according to UniProt)
  • 2,3-saturated fatty acid + H2O2 --> 3-hydroxy fatty acid + H2O (according to UniProt)

    • Protein family

  • cytochrome P450 family (according to UniProt)

    • Structure

  • 2ZQJ 1IZOPubMed

    • Expression and Regulation

    Operons

    Genes
    cypC
    Description
    (according to DBTBS) null

    Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

    • Regulation

  • induced by stress (SigB) PubMed
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    Biological materials

    Mutant

  • MGNA-B961 (ybdT::erm), available at the NBRP B. subtilis, Japan
  • BKE02100 (cypC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGCTTACCCTGCCTTC, downstream forward: _UP4_TAAATTAAAAAGCTCTCTTC
  • BKK02100 (cypC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGCTTACCCTGCCTTC, downstream forward: _UP4_TAAATTAAAAAGCTCTCTTC

    • References

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