SubtiBank SubtiBank
cwlC
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cwlC

N-acetylmuramoyl-L-alanine amidase
Locus
BSU_17410
Isoelectric point
5.94
Molecular weight
27.01 kDa
Protein length
255 aa Sequence Blast
Gene length
768 bp Sequence Blast
Function
mother cell lysis
Product
N-acetylmuramoyl-L-alanine amidase
Essential
no
E.C.
3.5.1.28
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall/ other
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

    • Gene

    Coordinates
    1,872,812 1,873,579

    The protein

    Catalyzed reaction/ biological activity

  • Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

    • Protein family

  • N-acetylmuramoyl-L-alanine amidase 3 family (according to UniProt)

    • Domains

  • contains an amidase_3 domain (like CwlD, LytC, YqiI, YrvJ)
  • MurNAc-LAA domain (aa 4-172) (according to UniProt)
  • SPOR domain (aa 180-254) (according to UniProt)

    • Structure

  • 1X60 (peptidoglycan binding domain) PubMed

    • Localization

  • secreted (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    cwlC
    Description
    PubMed

    Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon

    • Regulation

  • expressed late during sporulation in the mother cell (SigK) PubMed
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    Biological materials

    Mutant

  • BKE17410 (cwlC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTCATCACCTCATTA, downstream forward: _UP4_TAGCCGAGACGGGGACGAGC
  • BKK17410 (cwlC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTCATCACCTCATTA, downstream forward: _UP4_TAGCCGAGACGGGGACGAGC

    • References

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