SubtiBank SubtiBank
ctpA
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

ctpA

carboxy-terminal processing protease, promotes DNA damage checkpoint recovery
Locus
BSU_19590
Isoelectric point
8.86
Molecular weight
50.98 kDa
Protein length
466 aa Sequence Blast
Gene length
1401 bp Sequence Blast
Function
unknown
Product
carboxy-terminal processing protease
Essential
no
Synonyms
yzbD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Proteolysis → Additional proteins involved in proteolysis
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    2,131,902 2,133,302

    Phenotypes of a mutant

  • deletion of CtpA and DdcP leads to accumulation of the checkpoint protein YneA PubMed
  • sensitivity to DNA damage PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • digestion of YneA PubMed
  • The enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala-|-Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala (according to UniProt).

    • Protein family

  • Peptidase s41a family (together with CtpB, according to Uniprot)

    • Paralogous protein(s)

  • CtpB

    • Domains

  • signal peptide (aa 1 - 36) PubMed
  • PDZ domain (aa 96- 174) PubMed
  • S41 peptidase domain PubMed
  • peptidoglycan binding domain (C-terminal) PubMed

    • Structure

  • 4C2D (CtpB, 42% identity) PubMed

    • Localization

  • cell membrane, with extracellular protease domain PubMed

    • Expression and Regulation

    Operons

    Genes
    ctpA
    Description
    PubMed
    view in new tab

    Biological materials

    Mutant

  • MGNA-A087 (ctpA::erm), available at the NBRP B. subtilis, Japan
  • 1A866 ( ctpA::cat), [Pubmed| ], available at BGSC
  • BKE19590 (ctpA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTAAACACCACCTTTTT, downstream forward: _UP4_TAAAAAAAACCATACGCGGC
  • BKK19590 (ctpA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTAAACACCACCTTTTT, downstream forward: _UP4_TAAAAAAAACCATACGCGGC

    • References

    Loading