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crh
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crh

Carbon-flux regulating HPr, formerly known as Catabolite repression HPr-like protein, control of flux through the harmful methylglyoxal pathway, minor cofactor of the CcpA transcription factor
Locus
BSU_34740
Isoelectric point
4.70
Molecular weight
9.19 kDa
Protein length
85 aa Sequence Blast
Gene length
258 bp Sequence Blast
Function
control of carbon flux
Product
carbon-flux regulating HPr
Essential
no
Synonyms
yvcM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of transcription factor (other than two-component system)
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

    • Gene

    Coordinates
    3,569,292 3,569,549

    The protein

    Protein family

  • HPr family (with PtsH, according to UniProt)

    • Paralogous protein(s)

  • PtsH

    • Domains

  • Hpr domain (aa 1-85) (according to UniProt)

    • Modification

  • phosphorylation on Ser46 by HprK PubMed

    • Structure

  • 2AK7 (dimeric Crh-Ser46-P) PubMed
  • 1ZVV (CcpA-Crh-DNA complex)
  • 2RLZ (dimer)
  • 1MU4

    • Additional information

  • Crh does not possess the phosphorylation site used for PtsI phosphotransfer (His-15 in PtsH), it can only be phosphorylated on Ser-46

    • Expression and Regulation

    Operons

    Genes
    nahA-yvcJ-glmR-whiA-crh-yvcN
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpA: repression, in CcpA regulon

    • Regulation

  • constitutive expression at both protein and RNA levels PubMed

    • Additional information

  • A ncRNA is predicted between 'yvcI' and 'trxB' PubMed
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    Biological materials

    Mutant

  • GP860 (Δcrh::aphA3) PubMed, available in Jörg Stülke's lab
  • QB7097 (spc), available in Jörg Stülke's lab
  • BKE34740 (Δcrh::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT, downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT
  • BKK34740 (Δcrh::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT, downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT

    • Expression vectors

  • pGP641 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Jörg Stülke's lab
  • pGP734 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Jörg Stülke's lab

    • LacZ fusion

  • see nahA

    • Two-hybrid system

  • crh, crh(Ser46Asp), crh(Ser46Ala) B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Anne Galinier, University of Marseille, France
  • Richard Brennan, Houston, Texas, USA Homepage

    • References

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