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clpC
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clpC

AAA unfoldase, ATPase subunit of the ClpC-ClpP protease, directs proteins phosphorylated on arginine residues to ClpP
Locus
BSU_00860
Isoelectric point
5.75
Molecular weight
89.93 kDa
Protein length
810 aa Sequence Blast
Gene length
2433 bp Sequence Blast
Function
protein degradation, positive regulator of autolysin (LytC and LytD) synthesis
Product
AAA unfoldase, ATPase subunit of the ClpC-ClpP protease
Essential
no
Synonyms
mecB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall/ other
  • Information processing → Protein synthesis, modification and degradation → Proteolysis → Additional proteins involved in proteolysis
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
  • Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
  • Lifestyles → Coping with stress → Heat shock proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

    • Gene

    Coordinates
    103,572 106,004

    Phenotypes of a mutant

  • inactivation of clpC reduces sporulation efficiency to 0.4% that of wild type cells; delayed entry into sporulation, defect in engulfment with reduced SigG activity, and production of small spores PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • ATPase/chaperone

    • Protein family

  • ClpA/ClpB family (with ClpE, according to UniProt)

    • Paralogous protein(s)

  • ClpE

    • Domains

  • AAA-ATPase PFAM
  • UVR domain (aa 417-452) (according to UniProt)

    • Modification

  • phosphorylated on Arg-5 and Arg-254 PubMed
  • phosphorylated on Arg-70 PubMed

    • Structure

  • 2K77 (N-terminal domain)
  • 3PXG, 3J3U (the MecA-ClpC complex) PubMed

    • Localization

  • cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heatshock, colocalization with ClpP Pubmed
  • forms foci coincident with nucleoid edges, usually near cell poles PubMed

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

    • Expression and Regulation

    Operons

    Genes
    ctsR-mcsA-mcsB-clpC-radA-disA
    Description
    PubMed

    Sigma factors

  • SigM: sigma factor, PubMed, in SigM regulon
  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed PubMed, in SigB regulon
  • SigF: sigma factor, PubMed, in SigF regulon

    • Regulatory mechanism

  • CtsR: repression, PubMed, in CtsR regulon
  • Spx: activation, PubMed, in Spx regulon

    • Regulation

  • expressed during germination and spore outgrowth PubMed
  • induction during diamide stress (Spx) PubMed
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    Biological materials

    Mutant

  • clpC::tet available from the Hamoen Lab
  • BP98 (clpC::spc), available in Fabian Commichau's lab PubMed
  • BKE00860 (clpC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCGTTCTGTAAATCTTCCAA, downstream forward: _UP4_TAATATAGAAGACGGAAATG
  • BKK00860 (clpC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCGTTCTGTAAATCTTCCAA, downstream forward: _UP4_TAATATAGAAGACGGAAATG

    • GFP fusion

  • C-terminal GFP fusions (single copy, also as CFP and YFP variants) available from the Hamoen Lab

    • Antibody

  • available in Ulf Gerth's and Jörg Stülke's labs

    • Labs working on this gene/protein

  • Leendert Hamoen, Newcastle University, UK homepage
  • Kürsad Turgay, Freie Universitt Berlin, Germany homepage

    • References

    Reviews

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    Original Publications

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