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cdaS
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cdaS

sporulation-specific diadenylate cyclase, synthesis of c-di-AMP
Locus
BSU_19430
Isoelectric point
5.37
Molecular weight
22.75 kDa
Protein length
207 aa Sequence Blast
Gene length
624 bp Sequence Blast
Function
synthesis of c-di-AMP
Product
sporulation-specific diadenylate cyclase
Essential
no
E.C.
2.7.7.85
Synonyms
yojJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Metabolism of signalling nucleotides
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

    • Gene

    Coordinates
    2,118,504 2,119,127

    Phenotypes of a mutant

  • a cdaA cdaS disA triple mutant is not viable on complex medium; however, the mutant grows at low potassium concentration (0.1 mM) PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • synthesis of c-di-AMP from two molecules of ATP PubMed
  • 2 ATP --> 3',3'-c-di-AMP + 2 diphosphate (according to UniProt)

    • Protein family

  • adenylate cyclase family (with CdaA, according to UniProt)

    • Paralogous protein(s)

  • CdaA

    • Domains

  • N-terminal autoinhibitory domain PubMed
  • C-terminal DAC domain involved in the synthesis of c-di-AMP PubMed
  • DAC domain (aa 63-205) (according to UniProt)

    • Structure

  • 2FB5 (the protein of B. cereus, 48% identity, 81% similarity)

    • Expression and Regulation

    Operons

    Genes
    cdaS
    Description
    PubMed

    Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon

    • Regulation

  • expressed late during sporulation in the forespore (SigG) PubMed
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    Biological materials

    Mutant

  • MGNA-B426 (yojJ::erm), available at the NBRP B. subtilis, Japan
  • GP983 (cdaS::ermC), available in Jörg Stülke's lab PubMed
  • GP1360 (cdaS::spc), available in Jörg Stülke's lab
  • GP2222 (cdaA::cat cdaS::ermC disA::tet), available in Jörg Stülke's lab, the mutant is only viable on minimal medium at low potassium concentration PubMed
  • BKE19430 (cdaS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTCCTGTTTAAATTTCC, downstream forward: _UP4_TAAAAATAATAAAAAGGGCC
  • BKK19430 (cdaS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTCCTGTTTAAATTTCC, downstream forward: _UP4_TAAAAATAATAAAAAGGGCC

    • Expression vectors

  • IPTG inducible expression of '''B. cereus' Strep-cdaS in E. coli'': pGP2593 (in pGP172), available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Jörg Stülke, University of Göttingen, Germany Homepage

    • References

    Reviews

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    Original publications

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