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cdaR
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cdaR

effector protein controlling CdaA diadenylate cyclase activity
Locus
BSU_01760
Isoelectric point
4.72
Molecular weight
52.78 kDa
Protein length
483 aa Sequence Blast
Gene length
1452 bp Sequence Blast
Function
regulation of c-di-AMP synthesis
Product
effector protein controlling CdaA diadenylate cyclase activity
Essential
no
Synonyms
ybbR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Metabolism of signalling nucleotides

    • Gene

    Coordinates
    197,027 → 198,478

    The protein

    Catalyzed reaction/ biological activity

  • stimulates CdaA activity upon protein-protein interaction PubMed

    • Domains

  • YbbR-like 1 domain (aa 55-135) (according to UniProt)
  • YbbR-like 2 domain (aa 143-228) (according to UniProt)
  • YbbR-like 3 domain (aa 237-316) (according to UniProt)
  • YbbR-like 4 domain (aa 329-394) (according to UniProt)

    • Structure

  • 2KPU (one YbbR domain, from Desulfitobacterium hafniense) PubMed

    • Localization

  • cell membrane, oriented towards the outside PubMed

    • Expression and Regulation

    Operons

    Genes
    cdaA-cdaR-glmM-glmS
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
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    Additional information

  • the mRNA is very stable (> 15 min) PubMed

    • Biological materials

    Mutant

  • GP999 (cdaR::cat), available in Jörg Stülke's lab
  • GP985 (cdaA-cdaR::cat), available in Jörg Stülke's lab
  • BKE01760 (ΔcdaR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAGCCCAGCGGTTGTTTA, downstream forward: _UP4_GAATAAAAAAGGAGCGATTA
  • BKK01760 (ΔcdaR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAGCCCAGCGGTTGTTTA, downstream forward: _UP4_GAATAAAAAAGGAGCGATTA

    • Expression vectors

  • IPTG inducible expression of Strep-cdaR in E. coli: pGP2565 (in pGP172), available in Jörg Stülke's lab
  • IPTG inducible expression of His-cdaR in E. coli: pGP2557 (in pET19b), available in Jörg Stülke's lab
  • GP2031: cdaR-Strep aphA3, chromosomal expression in B. subtilis, for SPINE, available in Jörg Stülke's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmids: pGP1989 and pGP1992.

    • Antibody

  • available in Jörg Stülke's lab

    • References

    Reviews

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    Original publications

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