SubtiBank SubtiBank
bglS
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

bglS

endo-beta-1,3-1,4 glucanase
Locus
BSU_39070
Isoelectric point
6.48
Molecular weight
27.12 kDa
Protein length
242 aa Sequence Blast
Gene length
729 bp Sequence Blast
Function
lichenan degradation
Product
endo-beta-1,3-1,4 glucanase
Essential
no
E.C.
3.2.1.73
Synonyms
bgl,licS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of other polymeric carbohydrates
  • Groups of genes → Secreted proteins

    • Gene

    Coordinates
    4,011,842 4,012,570

    The protein

    Catalyzed reaction/ biological activity

  • Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds (according to UniProt)

    • Protein family

  • glycosyl hydrolase 16 family (single member, according to UniProt)

    • Domains

  • GH16 domain (aa 29-242) (according to UniProt)

    • Structure

  • 3O5S

    • Localization

  • extracellular (signal peptide) PubMed

    • Expression and Regulation

    Operons

    Genes
    bglS
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • LicT: antitermination, via binding to an RNA switch, in LicT regulon

    • Regulation

  • expressed in the stationary phase (temporal activation) PubMed
    • view in new tab

    Genes
    licT-bglS
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulation

  • expressed in the stationary phase (temporal activation) PubMed
    • view in new tab

    Genes
    yxiP-licT-bglS
    Description
    PubMed
    view in new tab

    Biological materials

    Mutant

  • GP427 (licT-bglS::erm), BGW7 (cat), both available in Jörg Stülke's lab
  • BKE39070 (bglS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGGCATTCCCCTTTC, downstream forward: _UP4_TAATGCCAAATGTGAAAGAG
  • BKK39070 (bglS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGGCATTCCCCTTTC, downstream forward: _UP4_TAATGCCAAATGTGAAAGAG

    • References

    Loading