SubtiBank SubtiBank
bglH
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bglH

phospho-beta-glucosidase
Locus
BSU_39260
Isoelectric point
4.96
Molecular weight
53.12 kDa
Protein length
469 aa Sequence Blast
Gene length
1410 bp Sequence Blast
Function
salicin utilization
Product
phospho-beta-glucosidase
Essential
no
E.C.
3.2.1.86
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of beta-glucosides

    • Gene

    Coordinates
    4,032,346 4,033,755

    The protein

    Catalyzed reaction/ biological activity

  • 6-phospho-β-D-glucosyl-(1→4)-D-glucose + H2O --> D-glucose + D-glucose 6-phosphate (according to UniProt)

    • Protein family

  • glycosyl hydrolase 1 family (according to UniProt)

    • Structure

  • 4IPL (from Streptococcus pneumoniae, 60% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    bglP-bglH-yxiE
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpA: repression, (CcpA binding site overlaps -35 region) PubMed, in CcpA regulon
  • LicT: anti-termination, via LicT-dependent RNA switch, lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HprK, in LicT regulon

    • Regulation

  • induced by salicin (LicT) PubMed

    • Additional information

  • An [ncRNA|search|antisense RNA] is predicted for 'bglP' PubMed
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    Biological materials

    Mutant

  • BKE39260 (bglH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGATCACCCCATTAG, downstream forward: _UP4_TGACAATTTTTTGAAAACTC
  • BKK39260 (bglH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGATCACCCCATTAG, downstream forward: _UP4_TGACAATTTTTTGAAAACTC

    • References

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