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bcaP
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bcaP

amino acid transporter, major transporter for isoleucine, valine and threonine, minor serine transporter
Locus
BSU_09460
Isoelectric point
9.73
Molecular weight
49.55 kDa
Protein length
465 aa Sequence Blast
Gene length
1398 bp Sequence Blast
Function
uptake of branched-chain amino acids, threonine, and serine
Product
amino acid transporter
Essential
no
Synonyms
yhdG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Transporters/ other → Amino acid transporters → APC superfamily
  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of branched-chain amino acids
  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of lysine/ threonine
  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of serine/ glycine/ alanine
  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of threonine/ glycine
  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of branched-chain amino acids
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    1,023,350 1,024,747

    Phenotypes of a mutant

  • no phenotype for the single mutant, the triple bcaP brnQ braB mutant is strongly impaired in the transport of isoleucine and valine at low concentrations PubMed
  • resistant to growth inhibition by high concentrations of threonine PubMed
  • resistant to 4-hydroxythreonine PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • high affinity uptake of isoleucine and valine PubMed
  • uptake of threonine PubMed
  • uptake of serine PubMed

    • Protein family

  • amino acid-polyamine-organocation (APC) superfamily (according to UniProt)

    • Paralogous protein(s)

  • MtrA

    • Structure

  • 5OQT (from Geobacillus kaustophilus, 60% identity) PubMed

    • Localization

  • cell membrane PubMed

    • Expression and Regulation

    Operons

    Genes
    bcaP
    Description
    PubMed

    Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon

    • Regulation:

  • repressed at high concentrations of branched-chain amino acids (CodY) PubMed
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    Biological materials

    Mutant

  • MGNA-B479 (yhdG::erm), available at the NBRP B. subtilis, Japan
  • BKE09460 (bcaP::erm, available in the BGSC and in Fabian Commichau's and Jörg Stülke's labs) PubMed
  • BKE09460 (bcaP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCCCTTCAACTCCCGA, downstream forward: _UP4_TAACCTTTTGATAAAGAGAG
  • BKK09460 (bcaP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCCCTTCAACTCCCGA, downstream forward: _UP4_TAACCTTTTGATAAAGAGAG

    • Expression vector

  • pGP2298 (expression with N-terminal His-tag from E. coli, in pWH844), available in Jörg Stülke's lab

    • References

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