SubtiBank SubtiBank
ansA
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

ansA

L-asparaginase
Locus
BSU_23580
Isoelectric point
4.45
Molecular weight
36.30 kDa
Protein length
329 aa Sequence Blast
Gene length
990 bp Sequence Blast
Function
asparagine degradation
Product
L-asparaginase
Essential
no
E.C.
3.5.1.1
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of asparagine/ aspartate

    • Gene

    Coordinates
    2,455,819 → 2,456,808

    The protein

    Catalyzed reaction/ biological activity

  • H2O + L-asparagine --> L-aspartate + NH4+ (according to UniProt)

    • Protein family

  • asparaginase 1 family (with AnsZ, according to UniProt)

    • Paralogous protein(s)

  • AnsZ

    • Domains

  • Asparaginase/glutaminase domain (aa 2-322) (according to UniProt)

    • Structure

  • 5OT0 (from Thermococcus kodakarensis, 43% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    ansA-ansB
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • AnsR: repression, PubMed, in AnsR regulon

    • Regulation

  • expressed in the presence of asparagine (AnsR) PubMed
    • view in new tab

    Genes
    ansA-ansB-mleN-mleA
    Description
    PubMed

    Regulation

  • ansA: expressed in the presence of asparagine (AnsR) PubMed
    • view in new tab

    Biological materials

    Mutant

  • GP1153 (ΔansA-ansB::ermC) available in Jörg Stülke's lab
  • BKE23580 (ΔansA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACCATGCACCTCTTCAC, downstream forward: _UP4_TAAAATGTAAAAACGATAAC
  • BKK23580 (ΔansA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACCATGCACCTCTTCAC, downstream forward: _UP4_TAAAATGTAAAAACGATAAC

    • LacZ fusion

  • pGP872 (in pAC5), available in Stülke lab

    • References

    Loading