SubtiBank SubtiBank
alsD
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alsD

acetolactate decarboxylase
Locus
BSU_36000
Isoelectric point
4.60
Molecular weight
28.65 kDa
Protein length
255 aa Sequence Blast
Gene length
768 bp Sequence Blast
Function
overflow metabolism
Product
acetolactate decarboxylase
Essential
no
E.C.
4.1.1.5
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Carbon core metabolism → Overflow metabolism
  • Groups of genes → Phosphoproteins → Phosphorylation on either a Ser, Thr or Tyr residue

    • Gene

    Coordinates
    3,708,799 3,709,566

    Phenotypes of a mutant

  • reduced acetoin production PubMed
  • impaired biofilm development PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • (2S)-2-acetolactate + H+ --> (R)-acetoin + CO2 (according to UniProt)

    • Protein family

  • alpha-acetolactate decarboxylase family (single member, according to UniProt)

    • Modification

  • phosphorylated on ser/ thr/ tyr PubMed, in vitro phosphorylated by PrkC on Ser-88 PubMed

    • Structure

  • 5XNE PubMed

    • Expression and Regulation

    Operons

    Genes
    alsS-alsD
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • AlsR: activation, in the presence of acetate PubMed, in AlsR regulon
  • Rex: repression, if the ratio NADH2/NAD is high PubMed, in Rex regulon
  • stringent response: positive regulation, due to presence of adenines at +1 and +2 positions of the transcript PubMed, in stringent response

    • Regulation

  • induction by acetate (AlsR) PubMed
  • expression is heterogeneous PubMed
  • expression of the operon is strongly induced at the beginning of biofilm formation PubMed
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    Biological materials

    Mutant

  • BKE36000 (alsD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTTCCCTTCACTCCTT, downstream forward: _UP4_TAAAAGAAAAAAAGAAAGCC
  • BKK36000 (alsD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTTCCCTTCACTCCTT, downstream forward: _UP4_TAAAAGAAAAAAAGAAAGCC

    • Expression vectors

  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP822, available in Jörg Stülke's lab, PubMed

    • References

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