SubtiBank SubtiBank
alaT
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

alaT

PLP-dependent L-alanine aminotransferase
Locus
BSU_31400
Isoelectric point
5.11
Molecular weight
42.15 kDa
Protein length
386 aa Sequence Blast
Gene length
1161 bp Sequence Blast
Function
biosynthesis of L-alanine
Product
PLP-dependent L-alanine aminotransferase
Essential
no
Synonyms
yugH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/acquisition of L- and D-alanine

    • Gene

    Coordinates
    3,225,772 3,226,932

    The protein

    Catalyzed reaction/ biological activity

  • pyruvate + glutamate --> L-alanine + 2-oxoglutarate reference

    • Protein family

  • class-I pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

    • Paralogous protein(s)

  • AspB, PatA

    • Cofactors

  • PLP

    • Structure

  • 1DJU (from Pyrococcus horikoshii, 47% identity) PubMed

    • Additional information

  • The gene is annotated in KEGG as an ortholog of N-succinyldiaminopimelate aminotransferase EC 2.6.1.17. It is annotated in Swiss-Prot as alanine transaminase. In MetaCyc the gene is marked as similar to aspartate
  • aminotransferase. No literature/experimental evidence supporting the annotation is available. PubMed

    • Expression and Regulation

    Operons

    Genes
    alaR-alaT
    Description
    PubMed

    Additional information

  • A ncRNA is predicted between yugI and alaT PubMed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A621 (yugH::erm), available at the NBRP B. subtilis, Japan
  • BKE31400 (alaT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATAGTCTGATAAATACGAAG, downstream forward: _UP4_TAAAAAAAGATACGAACCTT
  • BKK31400 (alaT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATAGTCTGATAAATACGAAG, downstream forward: _UP4_TAAAAAAAGATACGAACCTT

    • References

    Loading