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accD
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accD

acetyl-CoA carboxylase (beta subunit)
Locus
BSU_29210
Isoelectric point
5.34
Molecular weight
28.73 kDa
Protein length
290 aa Sequence Blast
Gene length
873 bp Sequence Blast
Function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
Product
acetyl-CoA carboxylase (beta subunit))
Essential
yes
E.C.
6.4.1.2
Synonyms
yttI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Lipid metabolism → Biosynthesis of lipids → Biosynthesis of fatty acids
  • Groups of genes → Essential genes
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

    • Gene

    Coordinates
    2,988,693 → 2,989,565

    Phenotypes of a mutant

  • essential PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • acetyl-CoA + N6-carboxybiotinyl-L-lysyl-[protein] --> malonyl-CoA + N6-biotinyl-L-lysyl-[protein] (according to UniProt)

    • Protein family

  • AccD/PCCB family (with YngE and YqjD, according to UniProt)

    • Domains

  • CoA carboxyltransferase N-terminal domain (aa 28-290) (according to UniProt)

    • Modification

  • phosphorylated on Arg-205 PubMed

    • Structure

  • 2F9I (from Staphylococcus aureus; equivalent to AccD-AccA, 64% identity) PubMed

    • Localization

  • Membrane-proximal (Spotty) PubMed

    • Expression and Regulation

    Operons

    Genes
    accD-accA
    Description
    PubMed

    Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon

    • Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed
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    Biological materials

    Expression vectors

  • purification from B. subtilis with an C-terminal Strep-tag, for SPINE, (in pGP382): pGP1724, available in Jörg Stülke's lab
  • pGP1026, N-terminal Strep-tag, based on pGP172, purification from E. coli, available in Jörg Stülke's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • FLAG-tag construct

  • GP1497 (mls, based on pGP1370), available in Jörg Stülke's lab

    • References

    Reviews

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    Original Publications

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