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eno
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eno

enolase, glycolytic/ gluconeogenic enzyme, universally conserved protein
locus
BSU_33900
pI
4.49
mw
46.42 kDa
protein length
430 aa Sequence Blast
gene length
1293 bp Sequence Blast
function
enzyme in glycolysis/ gluconeogenesis
product
enolase
essential
Yes
ec
4.2.1.11
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]
  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.5|Effectors of RNA degradation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]

    • Gene

    Coordinates
    3,476,555 3,477,847

    Phenotypes of a mutant

  • no growth on LB, requires glucose and malate [Pubmed|23420519]
  • essential according to Kobayashi et al. on LB [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • (2R)-2-phosphoglycerate --> H2O + phosphoenolpyruvate (according to UniProt)

    • Protein family

  • Enolase family (single member, according to UniProt)

    • Kinetic information

  • reversible Michaelis-Menten [Pubmed|25885]

    • [SW|Domains]

  • substrate binding domain (366369)

    • Modification

  • phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 [Pubmed|17218307,16493705,17726680]

    • [SW|Cofactors]

  • Mg2+

    • Effectors of protein activity

  • Inhibited by EDTA [Pubmed|25885]

    • Structure

  • [PDB|4A3R] [Pubmed|22198292]
  • [PDB|1W6T] (from ''Streptococcus pneumoniae'') [Pubmed|15476816]

    • [SW|Localization]

  • cytoplasm [Pubmed|16479537,27708634]
  • membrane associated [Pubmed|18763711]
  • aggregates at the cel poles of some cells [pubmed|29439991]
  • exported, this requires a long, unbent helix (from A108 to L126) [Pubmed|15003462,21856851]

    • Additional information

  • Enolase is a [SW|moonlighting proteins|moonlighting protein] [Pubmed|19193632]
  • There are indications that this enzyme is an octamer [Pubmed|25885]
  • [SW|universally conserved protein]
  • extensive information on the structure and enzymatic properties of Eno can be found at [http://www.proteopedia.org/wiki/index.php/Enolase Proteopedia]
  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Biological materials

    Mutant

  • GP594 (''eno''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP698 (''eno''-''[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]

    • Expression vectors

  • pGP1426 (expression of ''[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP1500 (expression of ''[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]'' and ''[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP563 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab, [pubmed|20389117]
  • pGP1276 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]
  • GP1215 (chromosomal ''eno''-''Strep'' fusion, ''spc''), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • see [gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]

    • GFP fusion

  • pHT315-yfp-eno, available in [SW|Mijakovic] lab
  • GP1700 (in [SW|pBP43]), expression of '' eno-GFP''::''spc'' under the native promoter, available in [SW|Jörg Stülke]'s lab [Pubmed|27708634]

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]

    • FLAG-tag construct

  • GP1214 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab [pubmed|21803996]

    • Antibody

  • available in [SW|Jörg Stülke]'s lab

    • labs

  • [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

    • References

    Reviews

  • 8994873

    • Subcellular localization of enolase

  • 16479537,18763711,20497499,15003462,21856851,24642254,27708634,29439991

    • Other original publications

  • 23420519,17726680,17218307,12850135,19193632,11489127,8021172,17505547,25885,20572937,15476816,9988532,21803996,22198292,15378759,28516784