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yqeK
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yqeK

symmetrically cleaving Ap4A hydrolase
locus
BSU_25630
pI
5.97
mw
21.14 kDa
protein length
186 aa Sequence Blast
gene length
561 bp Sequence Blast
function
degradation of Ap4A
product
Ap4A hydrolase
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.4|Nucleotide metabolism/ other]
  • Gene

    Coordinates
    2,643,215 2,643,775

    Phenotypes of a mutant

  • increased levels of Ap4A [pubmed|32152217]
  • The protein

    Catalyzed reaction/ biological activity

  • symmetric cleavage of Ap4A [pubmed|32152217]
  • [SW|Domains]

  • [SW|HD domain] (aa 18-132) (according to UniProt)
  • Structure

  • [PDB|3CCG] (the protein from ''Clostridium acetobutylicum'', 46% identity, 76% similarity)
  • Expression and Regulation

    Operons

    genes
    [gene|722FD63050EED803C859FFD61338C02D5BB4CC1C|yqeG]-[gene|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH]-[gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]-[gene|7774FC36BAEAD33EDD48CB77DA0B5E7E0EA38CA2|yqeI]-[gene|AA4BB1F5B58371D536A6BBA86F6ECB5E5BFB12A8|nadD]-[gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]-[gene|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|rsfS]-[gene|7F63251ABC07F6C11C57B62CBB948240B08D0181|yqeM]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • BKE25630 ([gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCTTCACGCATGCAAGTG, downstream forward: _UP4_AGCTGAACAGGAGGAATTTG
  • BKK25630 ([gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCTTCACGCATGCAAGTG, downstream forward: _UP4_AGCTGAACAGGAGGAATTTG
  • References

  • 15175311,22383849,32152217