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nudF

involved in isopentenol (isoprenoid) biosynthesis, has RNA pyrophosphohydrolase activity

locus

BSU_23610

4.59

20.84 kDa

protein length

185 aa Sequence Blast

gene length

558 bp Sequence Blast

function

isoprenoid biosynthesis

product

unknown

essential

3.6.1.13

synonyms

yqkG

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]

Gene

Coordinates

2,458,509 → 2,459,066

The protein

Catalyzed reaction/ biological activity

ADP-D-ribose + H₂O --> AMP + D-ribose 5-phosphate + 2 H⁺ (according to UniProt)

Protein family

[SW|Nudix hydrolase] (according to UniProt)

[SW|Domains]

[SW|Nudix hydrolase domain] (aa 40-171) (according to UniProt)

Structure

[PDB|1V8I] (from ''Thermus thermophilus'', 38% identity) [Pubmed|15210687]

Expression and Regulation

Operons

genes

[gene|CF38BE04F04919BB304789E9B3FE489385E468E6|nudF]-[gene|9D05B104000EED08FB62D6F362FC5E9C2A5354D9|yqxK]

description

[Pubmed|22383849]

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Biological materials

Mutant

MGNA-C410 (yqkG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2408 NBRP B. subtilis, Japan]

BKE23610 (Δ[gene|CF38BE04F04919BB304789E9B3FE489385E468E6|nudF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCATCTCCCGTTC, downstream forward: _UP4_AAAGAAGCGCTCCAAGCACA

BKK23610 (Δ[gene|CF38BE04F04919BB304789E9B3FE489385E468E6|nudF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCATCTCCCGTTC, downstream forward: _UP4_AAAGAAGCGCTCCAAGCACA

References

17693564,10542272,15210687,31740579