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ptsA
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ptsA

[category|SW 1.2.2|PTS] EIIA component
locus
BSU_22230
pI
5.29
mw
17.79 kDa
protein length
168 aa Sequence Blast
gene length
507 bp Sequence Blast
function
uptake and phosphorylation of maltose, N-acetylglucosamine, sucrose, and trehalose
product
[category|SW 1.2.2|PTS] EIIA component
essential
no
synonyms
ypqE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    2,336,933 2,337,439

    The protein

    Protein family

  • [category|SW 1.2.2|PTS] permease, glucose family [Pubmed|10627040]

    • [SW|Domains]

  • [SW|PTS EIIA domain] type-1 (aa 36-140) (according to UniProt)

    • Structure

  • [PDB|1AX3] (IIA domain of [protein|B5E7EB475434E96786C577AE709A21BD702733D8|PtsG], 46% identity) [pubmed|9593197]

    • Expression and Regulation

    Operons

    genes
    [gene|06165870CB68E9C2F1546943CEA668C7790A9647|ptsA]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|30038046], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • MGNA-A477 (ypqE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/477 NBRP B. subtilis, Japan]
  • QB6099 (aphA3), available in [SW|Jörg Stülke]'s lab [Pubmed|10627040]
  • BKE22230 (''ypqE''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
  • GP1863 (''ypqE''::''ermC'') (available in [SW|Jörg Stülke]'s lab)
  • BKE22230 ([gene|06165870CB68E9C2F1546943CEA668C7790A9647|ptsA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTATTTTCTCCCTTCT, downstream forward: _UP4_TAAGCAGGGCGTATGCCTTG
  • BKK22230 ([gene|06165870CB68E9C2F1546943CEA668C7790A9647|ptsA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTATTTTCTCCCTTCT, downstream forward: _UP4_TAAGCAGGGCGTATGCCTTG

    • References

  • 10627040,30038046,9593197