SubtiBank SubtiBank
yokF
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yokF

similar to micrococcal nuclease
locus
BSU_21610
pI
8.40
mw
32.85 kDa
protein length
296 aa Sequence Blast
gene length
891 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.2|SP-beta prophage]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    2,279,977 2,280,867

    The protein

    Paralogous protein(s)

  • [protein|91E6B4971F22B5A5F964590F8B14D6C73A4C4840|YncB]
  • [SW|Domains]

  • TNase-like domain (aa 66-199) (according to UniProt)
  • Structure

  • [PDB|4I65] (from Staphylococcus aureus, corresponds to aa 71 ... 198 of YncB, 57% identity)
  • Expression and Regulation

    Operons

    genes
    [gene|27F32FB0F96276325ED93710DF77B949658D728D|yokF]-[gene|C4C5A7CCD4B02EC846A40C52574A1092D5A95F68|yokE]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • BKE21610 ([gene|27F32FB0F96276325ED93710DF77B949658D728D|yokF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTCATTTCCCCTTTTA, downstream forward: _UP4_TGTGAACGATGAAAGGAGAT
  • BKK21610 ([gene|27F32FB0F96276325ED93710DF77B949658D728D|yokF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTCATTTCCCCTTTTA, downstream forward: _UP4_TGTGAACGATGAAAGGAGAT