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thiU
thiamine [SW|ECF transporter] (binding protein)
product
thiamine [SW|ECF transporter] (binding protein)
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter] → [category|SW 1.2.3.3|Class I ECF transporter][category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
1,391,040 1,391,642
The protein
Structure
[PDB|1SBR] (complex with thiamine), [PDB|1S99][SW|Localization]
membrane associated (via [protein|9AF27E4CE34B5C4036840A27D93B13213E3324DD|ThiV]-[protein|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|ThiX]) [Pubmed|16291685]Expression and Regulation
Operons
genes
[gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]-[gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]-[gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]
description
[Pubmed|16291685]
regulatory mechanism
[protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: [SW|RNA switch], via [SW|RNA switch], in [regulon|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]regulation
repressed by thiamine ([protein|search|Thi-box]) [Pubmed|12376536]view in new tabBiological materials
Mutant
MGNA-A757 (ykoF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/757 NBRP B. subtilis, Japan]BKK13240 ([gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTCCGACCTCCTGTT, downstream forward: _UP4_AGAAAAAACAGAAAGCAGGGReferences
16291685,12376536,15451668 
