SubtiBank SubtiBank
yjgC
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yjgC

general stress protein, formate dehydrogenase, important for survival of salt and ethanol stress
locus
BSU_12160
pI
5.18
mw
109.58 kDa
protein length
985 aa Sequence Blast
gene length
2958 bp Sequence Blast
function
unknown
product
formate dehydrogenase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.4|Electron transport/ other/ based on similarity]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

    • Gene

    Coordinates
    1,285,591 → 1,288,548

    The protein

    Protein family

  • C-terminal part: [SW|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|YrhE]

    • [SW|Domains]

  • 2Fe-2S ferredoxin-type domain (aa 3-79) (according to UniProt)
  • 4Fe-4S His(Cys)3-ligated-type domain (aa 79-119) (according to UniProt)
  • 2 [SW|4Fe-4S ferredoxin-type domain]s (aa 139-170, aa 182-211) (according to UniProt)
  • [SW|4Fe-4S Mo/W bis-MGD-type domain] (aa 258-314) (according to UniProt)

    • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]

    • Structure

  • [PDB|2FUG] (from Thermus thermophilus, 23% identity) [pubmed|16469879]

    • Expression and Regulation

    Operons

    genes
    [gene|E54F39E72DC4881E886159607C2B424A41DE0D01|yjgC]-[gene|01C43D05A53BF4B0F505D9C9CCAAFA1AF9E94F87|yjgD]
    description
    [Pubmed|11544224]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|11544224,15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

    • regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [Pubmed|11544224,15805528]
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    Biological materials

    Mutant

  • MGNA-A354 (yjgC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/354 NBRP B. subtilis, Japan]
  • BKE12160 (Δ[gene|E54F39E72DC4881E886159607C2B424A41DE0D01|yjgC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCCATTCCCCTTTCTT, downstream forward: _UP4_GCTGTGAAAAGGGGGATGGG
  • BKK12160 (Δ[gene|E54F39E72DC4881E886159607C2B424A41DE0D01|yjgC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCCATTCCCCTTTCTT, downstream forward: _UP4_GCTGTGAAAAGGGGGATGGG

    • References

  • 15805528,11544224,16469879