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thiL

thiamine-monophosphate kinase

locus

BSU_05900

5.04

35.73 kDa

protein length

325 aa Sequence Blast

gene length

978 bp Sequence Blast

function

biosynthesis of thiamine

product

thiamine-monophosphate kinase

essential

2.7.4.16

synonyms

ydiA,ydxA

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]

Gene

Coordinates

640,662 → 641,639

The protein

Catalyzed reaction/ biological activity

ATP + thiamine phosphate --> ADP + thiamine diphosphate (according to UniProt)

Protein family

thiamine-monophosphate kinase family (single member, according to UniProt)

Structure

[PDB|1VQV] (from ''Aquifex aeolicus'', 33% similarity, 51% identity)

Expression and Regulation

Operons

genes

description

[Pubmed|22383849]

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Biological materials

Mutant

MGNA-C197 (ydiA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2195 NBRP B. subtilis, Japan]

BKE05900 (Δ[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACCAAAACCTCCTTT, downstream forward: _UP4_TTTAAATAAGACGGGTGACT

BKK05900 (Δ[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACCAAAACCTCCTTT, downstream forward: _UP4_TTTAAATAAGACGGGTGACT

References

Reviews

19348578

Original publications

16291685,22383849