SubtiBank SubtiBank
hxlB
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hxlB

6-phospho-3-hexuloisomerase
locus
BSU_03450
pI
5.79
mw
19.82 kDa
protein length
185 aa Sequence Blast
gene length
558 bp Sequence Blast
function
ribulose monophosphate pathway for formaldehyde fixation
product
6-phospho-3-hexuloisomerase
essential
no
ec
5.3.1.27
synonyms
yckF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

    • Gene

    Coordinates
    374,603 → 375,160

    The protein

    Catalyzed reaction/ biological activity

  • D-arabino-hex-3-ulose 6-phosphate --> β-D-fructose 6-phosphate (according to UniProt)

    • Protein family

  • SIS family (single member, according to UniProt)

    • [SW|Domains]

  • [SW|SIS domain] (aa 29-172) (according to UniProt)

    • Structure

  • [PDB|1M3S] [pubmed|15363790]

    • Expression and Regulation

    Operons

    genes
    [gene|BF480721B89661BFFE6632BB10C5F4DC29951784|hxlA]-[gene|FB0CEA291CA4EC5FB8410050E61B89EDBA1771F9|hxlB]
    description
    [Pubmed|10572115]

    regulatory mechanism

  • [protein|D9A187961CFB9496A6712E9E48AAD384357A3E1C|HxlR]: activation, [Pubmed|10572115], in [regulon|D9A187961CFB9496A6712E9E48AAD384357A3E1C|HxlR regulon]

    • regulation

  • induction by formaldehyde ([protein|search|HxlR]) [Pubmed|10572115]
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    Biological materials

    Mutant

  • MGNA-C056 (yckF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2054 NBRP B. subtilis, Japan]
  • BKE03450 (Δ[gene|FB0CEA291CA4EC5FB8410050E61B89EDBA1771F9|hxlB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTATTCAGTCGTTTTCATCG, downstream forward: _UP4_TAGCATCACACAACCGGCCT
  • BKK03450 (Δ[gene|FB0CEA291CA4EC5FB8410050E61B89EDBA1771F9|hxlB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTATTCAGTCGTTTTCATCG, downstream forward: _UP4_TAGCATCACACAACCGGCCT

    • References

  • 10572115,11468398,16428816,19170879,15363790